dr nevan krogan rrid addgene 141383 (Addgene inc)
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dr nevan krogan rrid addgene 141383
Dr Nevan Krogan Rrid Addgene 141383, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nevan+krogan/pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro+(Plasmid+%23141383)/pmc12754350-31-5-8
Average 93 stars, based on 10 article reviews
Dr Nevan Krogan Rrid Addgene 141383, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nevan+krogan/pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro+(Plasmid+%23141383)/pmc12754350-31-5-8
Average 93 stars, based on 10 article reviews
dr nevan krogan rrid addgene 141383 - by Bioz Stars,
2026-10
93/100 stars
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Modification:Article Title: Nuclear pore permeability and fluid flow are modulated by its dilation state. Article Snippet: .. HEK293 Flp-In T-REx cells (Invitrogen) cells were grown in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) with 10% fetal bovine serum and penicillin-streptomycin in a 5% CO2 humidified incubator at 37 C. pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro was a gift from Plasmid Preparation:Article Title: Nuclear pore permeability and fluid flow are modulated by its dilation state. Article Snippet: .. HEK293 Flp-In T-REx cells (Invitrogen) cells were grown in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) with 10% fetal bovine serum and penicillin-streptomycin in a 5% CO2 humidified incubator at 37 C. pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro was a gift from Article Title: Inhalable SARS-CoV-2 Mimetic Particles Induce Pleiotropic Antigen Presentation Article Snippet: .. The purified pEGFP vector was a generous gift from the laboratory of Dr. Angela Pannier at the University of Nebraska, Lincoln, Department of Biological Systems Engineering. pCMV14–3X-Flag-SARS-CoV-2 S was a gift from Zhaohui Qian (Addgene plasmid #145780; http://n2t.net/addgene:145780 ; RRID: Addgene_145780). pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro was a gift from Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids Article Snippet: Viral stocks were stored at −80 °C and titrated via TCID 50 (50% tissue culture infective dose) or plaque-forming assays. .. Expression plasmids for SARS-CoV-2 open reading frames and an eGFP control in the pLVX-EF1alpha-2xStrep-IRES-Puro vector 5 , were gifts from Article Title: Subcellular localization of SARS-CoV-2 E and 3a proteins along the secretory pathway Article Snippet: Insert containing clones were confirmed by restriction digest analysis and DNA sequencing. pAAV EF1a CoV2 Orf3a-2xStrep (Addgene, #213542) was constructed by inserting a codon optimized SARS-CoV-2 Orf3a-2xstrep (amplified from Addgene, #141383) into a pAAV EF1a backbone. .. The Addgene plasmid#141383 pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro was a gift from Article Title: Efficacy and safety of metabolic interventions for the treatment of severe COVID-19: in vitro, observational, and non-randomized open-label interventional study Article Snippet: .. Generation lentiviral SARS-CoV-2 constructs Plasmids encoding the SARS- CoV- 2 open- reading frames (ORFs) and eGFP control are a kind gift of Article Title: An iron-sulfur cluster in the zinc-binding domain of the SARS-CoV-2 helicase modulates its RNA-binding and -unwinding activities. Article Snippet: .. Recombinant expression of C- terminally Strep II- nsp13 in Expi293F, 293T cells was obtained from pLVX- EF1alpha- SARS- CoV- 2- nsp13- 2xStrep- IRES- Puro, a gift from Clone Assay:Article Title: Nuclear pore permeability and fluid flow are modulated by its dilation state. Article Snippet: .. HEK293 Flp-In T-REx cells (Invitrogen) cells were grown in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) with 10% fetal bovine serum and penicillin-streptomycin in a 5% CO2 humidified incubator at 37 C. pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro was a gift from Sequencing:Article Title: Nuclear pore permeability and fluid flow are modulated by its dilation state. Article Snippet: .. HEK293 Flp-In T-REx cells (Invitrogen) cells were grown in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) with 10% fetal bovine serum and penicillin-streptomycin in a 5% CO2 humidified incubator at 37 C. pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro was a gift from Purification:Article Title: Inhalable SARS-CoV-2 Mimetic Particles Induce Pleiotropic Antigen Presentation Article Snippet: .. The purified pEGFP vector was a generous gift from the laboratory of Dr. Angela Pannier at the University of Nebraska, Lincoln, Department of Biological Systems Engineering. pCMV14–3X-Flag-SARS-CoV-2 S was a gift from Zhaohui Qian (Addgene plasmid #145780; http://n2t.net/addgene:145780 ; RRID: Addgene_145780). pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro was a gift from Expressing:Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids Article Snippet: Viral stocks were stored at −80 °C and titrated via TCID 50 (50% tissue culture infective dose) or plaque-forming assays. .. Expression plasmids for SARS-CoV-2 open reading frames and an eGFP control in the pLVX-EF1alpha-2xStrep-IRES-Puro vector 5 , were gifts from Article Title: An iron-sulfur cluster in the zinc-binding domain of the SARS-CoV-2 helicase modulates its RNA-binding and -unwinding activities. Article Snippet: .. Recombinant expression of C- terminally Strep II- nsp13 in Expi293F, 293T cells was obtained from pLVX- EF1alpha- SARS- CoV- 2- nsp13- 2xStrep- IRES- Puro, a gift from Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids. Article Snippet: Gateway-compatible entry plasmids (pDONR207, 223) for S (Addgene 152988), NSP3 (Addgene 141257), and NSP16 (Addgene 141269) were from Frederick Roth (Supplementary Note 1). .. Expression plasmids for SARS-CoV-2 open reading frames and an eGFP control in the pLVX-EF1alpha-2xStrepIRES-Puro vector5, were gifts from Control:Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids Article Snippet: Viral stocks were stored at −80 °C and titrated via TCID 50 (50% tissue culture infective dose) or plaque-forming assays. .. Expression plasmids for SARS-CoV-2 open reading frames and an eGFP control in the pLVX-EF1alpha-2xStrep-IRES-Puro vector 5 , were gifts from Article Title: Efficacy and safety of metabolic interventions for the treatment of severe COVID-19: in vitro, observational, and non-randomized open-label interventional study Article Snippet: .. Generation lentiviral SARS-CoV-2 constructs Plasmids encoding the SARS- CoV- 2 open- reading frames (ORFs) and eGFP control are a kind gift of Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids. Article Snippet: Gateway-compatible entry plasmids (pDONR207, 223) for S (Addgene 152988), NSP3 (Addgene 141257), and NSP16 (Addgene 141269) were from Frederick Roth (Supplementary Note 1). .. Expression plasmids for SARS-CoV-2 open reading frames and an eGFP control in the pLVX-EF1alpha-2xStrepIRES-Puro vector5, were gifts from Construct:Article Title: Subcellular localization of SARS-CoV-2 E and 3a proteins along the secretory pathway Article Snippet: Insert containing clones were confirmed by restriction digest analysis and DNA sequencing. pAAV EF1a CoV2 Orf3a-2xStrep (Addgene, #213542) was constructed by inserting a codon optimized SARS-CoV-2 Orf3a-2xstrep (amplified from Addgene, #141383) into a pAAV EF1a backbone. .. The Addgene plasmid#141383 pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro was a gift from Article Title: Efficacy and safety of metabolic interventions for the treatment of severe COVID-19: in vitro, observational, and non-randomized open-label interventional study Article Snippet: .. Generation lentiviral SARS-CoV-2 constructs Plasmids encoding the SARS- CoV- 2 open- reading frames (ORFs) and eGFP control are a kind gift of Amplification:Article Title: Subcellular localization of SARS-CoV-2 E and 3a proteins along the secretory pathway Article Snippet: Insert containing clones were confirmed by restriction digest analysis and DNA sequencing. pAAV EF1a CoV2 Orf3a-2xStrep (Addgene, #213542) was constructed by inserting a codon optimized SARS-CoV-2 Orf3a-2xstrep (amplified from Addgene, #141383) into a pAAV EF1a backbone. .. The Addgene plasmid#141383 pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro was a gift from Recombinant:Article Title: An iron-sulfur cluster in the zinc-binding domain of the SARS-CoV-2 helicase modulates its RNA-binding and -unwinding activities. Article Snippet: .. Recombinant expression of C- terminally Strep II- nsp13 in Expi293F, 293T cells was obtained from pLVX- EF1alpha- SARS- CoV- 2- nsp13- 2xStrep- IRES- Puro, a gift from |